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B059 |
TRYPTOSE SULPHITE NEOMYCIN AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Tryptose Yeast extract Sodium sulphite Ferric citrate Neomycin sulphate Polymixin B sulphate Agar |
15.00 10.00 1.00 0.50 0.05 0.02 13.50 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 40 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense in screw capped containers. Autoclave with caps loose at 12 lbs pressure (118°C) for 12 minutes. Close the caps while the medium is still hot. 5ml. of sterile buffered thioglycollate solution may be added to every 200 ml. of medium if desired.The buffered aqueous thioglycollate solution contains 35 ml. buffer mixture (5.7% dipotassium phosphate and 28% sodium carbonate) and 15 ml. sodium thioglycollate solution (13.3%). |
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