B053

 MANNITOL SALT AGAR

Formula 

B053 

Ingredients 

gms/lit. 

Proteose peptone

Beef extract 

Sodium chloride 

D-Mannitol 

Phenol red 

Agar 

10.00 

1.00 

75.00 

10.00 

0.025 

15.00 

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 111gms. of (B053) in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. If desired, add 5% v/v Egg yolk Emulsion to (B053). Mix well & dispense as desired.

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