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B048 |
TRIPLE SUGAR IRON AGAR |
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Formula |
B048 |
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Ingredients |
gms/lit. |
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Pepetic digest of animal tissue Casein enzymic hydrolysate Yeast extract Beef extract Lactose Sucrose Dextrose Sodium chloride Ferrous sulphate Sodium thiosulphate Phenol red Agar |
10.00 10.00 3.00 3.00 10.00 10.00 1.00 5.00 0.20 0.30 0.024 12.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 65 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Mix well and distribute into test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the medium to set in sloped form with a butt about 1 inch long . |
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