|
B211 |
KF STREPTOCOCCAL BROTH |
|
|
Formula |
||
|
Ingredients |
gms/lit. |
|
|
Peptone, special Yeast extract Sodium chloride Sodium glycerophosphate Sodium carbonate Maltose Lactose Sodium azide Phenol red |
10.00 10.00 5.00 10.00 0.636 20.00 1.00 0.40 0.018 |
|
|
Final pH (at 25°C) : 7.2 + 0.2 |
||
|
Directions |
||
|
Suspend 57 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes. If desired for use in the membrane filter technique, one ml of 1% Triphenyl Tetrazolium Chloride (TTC-BF044) may be added to each 100 ml of sterile cooled broth. |
||