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B172 |
DIAGNOSTIC SENSITIVITY TEST AGAR (D.S.T. AGAR) |
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Formula |
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Ingredients |
gms/lit. |
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Proteose peptone Veal iinfusion solids Dextrose Sodium chloride Disodium phosphate Sodium acetate Adenine sulphate Guanine hydrochloride Uracil Xanthine Aneurine Agar |
10.00 10.00 2.00 3.00 2.00 1.00 0.01 0.01 0.01 0.01 0.00002 15.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 43 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. For blood agar, cool the base to 50°C & add 7% v/v sterile defibrinated horse blood aseptically. Mix well with gentle rotation & pour into sterile petri plates. |
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